柯莎研究员

简历：

•         1993-1996年英国剑桥大学化学系获博士学位
          1996-1997年英国剑桥大学化学系博士后
          1997-2000年英国剑桥大学化学系Research Fellow
          2000-2003年中国科学院生物物理研究所，英国皇家学会 Research Fellow
          2003至今 中国科学院生物物理研究所研究组长
          现任研究员，博士生导师，中科院生物物理研究所学术交流与合作委员会委员

研究组工作摘要：

        酵母Ure2蛋白在体内具有Prion性质，在体外易形成淀粉样纤维，是安全的研究Prion疾病的模型蛋白。Ure2蛋白含有重复序列的N-端与Prion性质密切相关，我们已对Ure2蛋白及其突变体的构象、折叠和淀粉样纤维形成性质进行了深入研究,发现Ure2蛋白N-端(Prion域)是无固定结构的柔性区域，不影响C-端的折叠，但对淀粉样纤维形成初期的“成核”过程起重要作用。我们正在研究分子伴侣等因素对Ure2聚集性质的影响，以在分子水平了解Prion形成的机制。

代表性论文：

1. Perrett S* & Jones GW*. Insights into the mechanism of prion propagation. CURR OPIN STRUC BIOL 18, 52-59 (2008). (Invited Review)

   Gao L, Zhuang J, Nie L, Zhang J, Gu N, Wang T, Perrett S* & Yan X*. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. NATURE NANOTECHNOL 2, 577-583 (2007).

   Shi, Y, Fan, DJ, Li, SX, Zhang, HJ, Perrett, S.& Zhou JM .Identification of a potential hydrophobic peptide binding site in the C-terminal arm of trigger factor. PROTEIN SCIENCE, 2007, 16: 1165-1175.

   Lian HY, Zhang H, Zhang ZR, Loovers HM, Jones GW, Rowling P, Itzhaki LS, Zhou JM & Perrett S*. Hsp40 interacts directly with the native state of the yeast prion protein Ure2 and inhibits formation of amyloid-like fibrils. J BIOL CHEM,  282, 11931-11940 (2007).

   Immel F1, Jiang Y1, Wang YQ, Marchal C, Maillet L, Perrett S* & Cullin C*. In vitro analysis of SpUre2p, a prion related protein, exemplifies the relationship between amyloid and prion. J BIOL CHEM 282, 7912-7920 (2007).

   Lian HY, Jiang Y, Zhang H, Jones GW & Perrett S*. The Yeast Prion Protein Ure2: Structure, Function and Folding. BBA 1764, 535-545 (2006). (Invited Review)

   Liu CP, Perrett S & Zhou JM*. Dimeric trigger factor stably binds folding-competent intermediates and cooperates with the DnaK-DnaJ-GrpE chaperone system to allow refolding. J BIOL CHEM 280, 13315-13320 (2005).

   Bai M, Zhou JM* & Perrett S*. The yeast prion protein Ure2 shows glutathione peroxidase activity in both native and fibrillar forms. J BIOL CHEM 279, 50025-50030 (2004).

   Jiang Y, Li H, Zhu L, Zhou JM* & Perrett S*. Amyloid Nucleation and Hierarchical Assembly of Ure2p Fibrils: Role of Asn/Gln Repeat and Non-repeat Regions of the Prion Domain. J BIOL CHEM 279, 3361-3369 (2004).

   Zhu L, Zhang XJ, Wang LY, Zhou JM* & Perrett S*. Relationship Between Stability of Folding Intermediates and Amyloid Formation for the Yeast Prion Ure2p: A Quantitative Analysis of the Effects of pH and Buffer System. J MOL BIOL 328, 235-254 (2003).

   Galani D, Fersht AR & Perrett S*. Folding of the Yeast Prion Protein Ure2: Kinetic Evidence for Folding and Unfolding Intermediates. J MOL BIOL 315, 213-227 (2002).

2. 电子邮件(E-mail )	sperrett(AT)sun5.ibp.ac.cn（请将(AT)替换为@，防止垃圾邮件）
   电话(Tel)	010-64856727
   房间号（Room No.)	3308